Effect of 5HT pretreatment on SERT Expression.

In CHO-SERT and CHO-(SERT+S56A) cells, the effect of 5HT pretreatment on the surface density of SERT proteins was tested at two different concentrations: at low (10 ��M) and high (100 ��M) 5HT. Twenty-four hour post-transfected cells were pretreated with 5HT and biotinylated with NHS-SS-biotin [18], [23], [26]. Intracellular SERT and biotinylated plasma membrane proteins were analyzed with W/B with SERT antibodies. The results of W/B analysis are the summary of combined data from three densitometric scans denoted as the percent change of SERT density in 5HT pretreated cells compared to untreated cells.