Hepatocyte-macrophage crosstalk via the PGRN-EGFR axis modulates ADAR1-mediated immunity in the liver -HCC scRNAseq

ADAR1 is thought to be an immune suppressor maintaining self-tolerance to endogenous nucleic acids. ADAR1 is often overexpressed in hepatocellular carcinoma tumors, and might contribute to immunosuppression and immune evasion of cancer cells. We performed single cell RNA sequencing on non-tumor and tumor HCC samples. Constructed libraries were sequenced on the HiSeq X platform. Over 150 million pairs of mappable reads for each sample were obtained. Transcriptomes of single cells isolated from non-tumor and tumor HCC samples were examined to investigate immunosuppression and association with cellular crosstalk mechanisms.