Dissecting the effects of sequence on transcription attenuation by the Restrictor complex [INSERT-Seq]

The eukaryotic genome is actively transcribed by RNA Polymerase II (RNAPII) to produce both messenger RNAs (mRNAs) and a repertoire of non-coding RNAs (ncRNAs). For genome integrity, RNAPII elongation and termination is tightly regulated to enable the full-length synthesis of mRNAs and the rapid turnover of many ncRNAs. The Restrictor Complex has recently been implicated in attenuating the expression of many ncRNAs, however the mechanism by which Restrictor suppresses gene expression remains unclear. Here, we address central questions regarding the specificity of Restrictor-mediated transcription attenuation. Overall design: INSERT-seq with steady-state-RNA-level read-out was performed for 9 samples: 3 biological replicate sets of siNT, siWDR82 and siZC3H4 treated cells.