Genome-wide atlas of promoters expression reveals contribution of transcribed regulatory elements to genetic control of disuse-mediated atrophy of skeletal muscles

Here we report genome wide estimation of expression profiles at promoter level in Soleus and EDL muscles in rat hindlimbs under unloading conditions. Samples were collected from rat hindlimbs at normal state, after unloading for 24 hours, 3 days, 7 days; recovery after 7 days unloading for 24 hours, 3 days, 7 days. Extracted total RNA were proceeded into cDNA and submitted to nAnTi-CAGE libraries preparation with subsequent sequencing on Illumina HiSeq2500 (SE, 50bp)