Transcriptome differences between mature luminal and luminal progenitor cells of the breast

In this study, we grew primary breast epithelial cells from breast biopsies of healthy donors, five each of African Ancestry, European ancestry and those who identify as Hispanic using epithelial cell reprogramming assay growth method. Cells grown were sorted into luminal progenitors (CD49F+/EpCAM+, labelled as PP cells) and mature luminal (CD49F-/EpCAM+, labelled as NP cells) by flow cytometry. Flow sorted cells were subjected to RNA-seq. Step1: grow primary cells from breast tissues of healthy donors, Step 2: Sort grown cells into mature luminal and luminal progenitor cells by flow cytometry, step 3: prepare RNA directly from the flow sorted cells and subject to RNA-seq