Whole-transcriptome sequencing of A549 cells and cisplatin-resistance A549/DPP cells

The aim of this study was to investigate the molecular mechanisms underlying cisplatin 14 (DDP) resistance in non-small cell lung cancer (NSCLC) cells by constructing a competing 15 endogenous RNA (ceRNA) network. The gene expression profiles of human lung adenocarcinoma DDP-resistant cell line (A549/DDP) and its progenitor (A549) were comparatively evaluated by whole-transcriptome sequencing. The differentially expressed genes (DEGs) were subjected to KEGG pathway analysis. The expression levels of mRNAs involved in several pathways associated with conferring DDP resistance to tumor cells were evaluated. The ceRNA network was constructed based on the mRNA expression levels and the sequencing data of miRNA and lncRNA. Several ceRNA regulatory relationships were validated