Relationship between quinone concentrations and ArcA phosphorylation.

Total ubiquinone content (nmoles/g), demethylmenaquinone content (nmoles/g), menaquinone content (nmoles/g) and ArcA phosphorylation (%) for MG1655 (Wild type) and its quinone mutants during exponential growth in Evan’s medium supplied with 50 mM glucose and 1% LB at 37°C under aerobic, anaerobic and anaerobic with 50 mM fumarate conditions. The amount of quinone (nmoles/g) is expressed in nanomoles per gram dry cell weight. The values represent the mean of measured values from biological triplicates with standard deviation. WT: K12-Wild type.