Additional file 2: Figure S1. of Functional characterization of Plasmodium berghei PSOP25 during ookinete development and as a malaria transmission-blocking vaccine candidate

Predicted B cell epitopes of the PSOP25 protein ( http://tools.iedb.org/bcell ). Below is the protein domain architecture of PSOP25 with signal peptide highlighted in red, low complexity in pink, and transmembrane region in blue. Figure S2. The isotype of anti-rPSOP25 mAb was identified by ELISA using by the SBA Clonotypingâ ˘ System-HRP. The data represent two separate experiments. Error bar shows meanâ +â standard deviation. Figure S3. Western blot analysis of P. berghei ookinete lysates with anti-rPSOP25 sera (PolyAb) and anti-rPSOP25 mAb. Lysates were subjected to electrophoresis under non-reducing conditions by SDS-PAGE. Pbs21 mAb was used as positive control; a control mouse serum (PBS) was used as negative control. (ZIP 2413 kb)