Results for normal human plasma (NHP) containing HIV-1 RNA diluted to 580, 290, 145, 73, and 0 (negative control) total copies in a 25 µl reaction.

Extracted genomic nucleic acid at 2.2 ng/µL was in all samples except the negative control and HIV-positive control. In this comparison, samples and controls assayed by the non-instrumented nucleic acid amplification (NINA)/nucleic acid lateral flow (NALF) system and the Stratagene system were aliquoted from the same sample extraction and handled identically up to amplification. Since the NINA heater does not have top-heating, oil was added to these samples for amplification to control for any condensation artifacts. The samples in the top-heated Stratagene were amplified as per the manufacturer’s instructions (no mineral oil) to reflect a standardized reference method.Results for normal human plasma (NHP) containing HIV-1 RNA diluted to 580, 290, 145, 73, and 0 (negative control) total copies in a 25 µl reaction.