Peel-and-Swiss Roll Biopsy: A Practical Technique for Tissue Sampling in Fragile Blistering Skin Conditions

1. Video demonstration of the peel-and-Swiss roll biopsy technique in a representative patient with pemphigus vulgaris, illustrating the atraumatic retrieval and preparation of a detached epidermal sheet for histopathological examination
2. Supplementary Figure 1: (a) Representative tissue section from a patient with Staphylococcal scalded skin syndrome (SSS), which shows a rolled-up, detached horny layer (100x); (b) High-power view shows mounds of parakeratosis, with focal cluster of acantholytic cells (400x).
3. Supplementary Figure 2: (a) Representative tissue sections from a patient with pemphigus vulgaris, showing “Swiss-roll” configuration at low-power magnification (100x); (b) A close-up view showing the roof of intraepidermal with distinct acantholysis (400x).