L1 Retrotransposon Sequencing in Schizophrenia - Study 1

Schizophrenia (SZ) is a devastating psychiatric disorder with substantial global clinical and economic implications. While SZ is marked by high heritability, discovery of common variants associated with even moderate risk for SZ has proven difficult. We used Restriction Enzyme Based Enriched L1 sequencing (REBELseq) to amplify L1 retrotransposons in gDNA isolated from dorsolateral prefrontal cortex NeuN+ neuronal nuclei from 63 SZ cases and 63 controls. Amplified libraries were sequenced on the Illumina HiSeq 4000 platform, and sequencing data was analyzed with the REBELseq bioinformatics pipeline.]]>
Schizophrenia: SZ cases were adult individuals who met DSM criteria for an SZ diagnosis. Subjects were excluded for causes of death causing acute neuronal damage or prolonged hypoxia (e.g. suicide by gunshot wound to the head or hanging). Controls: Adult individuals who died of acute causes and had no history of psychiatric disorders. Subjects were excluded for causes of death causing acute neuronal damage or prolonged hypoxia (e.g. suicide by gunshot wound to the head or hanging).]]>