Allowable number of linker conformations in the absence and presence of fusion proteins*.

*Note that while the absolute numbers of conformations are dependent on the parameters used for modeling, the relative magnitudes can be directly compared. Relative magnitudes (in parentheses) are calculated by dividing the value of Protein+crmCherry by the value of Protein with linker alone (no tag).**For constructs of direct fusion, the last 2 amino acid residues at the C-terminus of TPP1 or NPC2 and the first 3 residues at the N-terminus of crmCherry were used for the analysis.