High-resolution mapping of transcribing RNAPIII by CRAC upon depletion of Nrd1 [Experiment 2]

Here we report high-resolution analyses of transcribing RNAPIII in either a wild-type (WT) background or a Nrd1 auxin-inducible degron (AID) strain in which Nrd1 can be rapidly depleted upon addition of the auxin analogue Indole-3-acetic acid (IAA). Nrd1 functions together with Sen1 in transcription termination at RNAPII-dependent non-coding genes. Here we show that depletion of Nrd1 does not affect transcription termination at RNAPIII-dependent genes, indicating that Nrd1 is not an RNAPIII transcription termination factor.本研究报道了在野生型（WT）背景或Nrd1生长素诱导降解（AID）菌株中，对转录状态下的RNA聚合酶III（RNAPIII）开展的高分辨率分析；该AID菌株中，添加生长素类似物吲哚-3-乙酸（IAA）即可快速耗竭Nrd1。Nrd1与Sen1协同参与RNA聚合酶II（RNAPII）依赖的非编码基因的转录终止过程。本研究结果显示，Nrd1耗竭不会影响RNA聚合酶III（RNAPIII）依赖基因的转录终止，表明Nrd1并非RNA聚合酶III（RNAPIII）的转录终止因子。