Construction of the axolotl cell landscape using combinatorial hybridization sequencing at single cell resolution [bulk RNA-seq]

We adopted a combinatorial hybridization based single-cell RNA-seq method to generate tissue based transcriptome atlas of adult axolotl and whole organism transcriptome atlas of larva axolotl. Gene expression profiling of over 1million single cells across 19 organs constructed the first adult axolotl cell atlas. Comparison between neoteny and metamorphosis organs revealed transcriptome heterogeneity of structural cells in different tissues and a sophisticated regulatory network. Furthermore, we described dynamic gene expression pattern during neotenic larva axolotl limb development. These data serve as a rich resource to explore molecular identity of axolotl as well as its metamorphosis. Bulk RNA sequencing datasets for seventeen adult neotenic axolotl tissues (brain, bladder, cloaca, eye, fore limb, hind limb, gill, gonad, heart, intestine, kidney, liver, lung, skin, spleen, stomach, tail) and fourteen adult metamorphosed axolotl tissues (bladder, brain, cloaca, eye, hind limb, heart, intestine, kidney, liver, lung, skin, spleen, stomach, tail). Single cell combinatorial hybridization RNA sequencing datasets for nineteen adult neotenic axolotl tissues (brain, bladder, cloaca, eye, fore limb, hind limb, gill, gonad, pancreas, prostate, heart, intestine, kidney, liver, lung, skin, spleen, stomach, tail), sixteen adult metamorphosed axolotl tissues ( bladder, brain, cloaca, eye, fore limb, hind limb, heart, intestine, kidney, liver, lung, skin, spleen, stomach, tail, gonad) and neotenic larva stage axolotl whole organism covering 5 stages including Day 30, Day 35, Day 45, Day 50, Day 70 postfertilization.