Large-scale genomic alterations identified in cancer patients on the course of cancer recovery with complementary Huaier treatment identified by total RNA- and small non-coding RNA-sequencing.

Tremetes robiniophila Murr (Huaier), has been well known by many literatures as a significant cancer complementary therapy. We have proved that a broad spectrum of anti-cancer effect of Huaier is based on the rescue of the disrupted Hippo signalling pathway, especially through the recovery in transcriptional dysregulation. We have further investigated the systematic molecular mechanism of cancer recovery as clinical research, using a systematic transcriptome analysis using genome-scale mRNA and small non-coding RNA sequencing. The obtained peripheral blood samples (from cancer patients as well as normal controls) were designated to be used for RNA extraction, construction of whole transcriptome library for sequencing on DNBSeq Platform, followed by whole transcriptome mapping. We have also performed total non-coding small RNAs sequencing on the same DNBSeq Platform, and the length of small RNAs were found to be between 18 to 30 nucleotides. Total 91 samples from 32 cancer patients were sequenced and analysed.