Effect of methanol fixation on single cell RNA sequencing of the murine dentate gyrus.
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https://www.ncbi.nlm.nih.gov/sra/SRP436652
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资源简介：
We evaluated the effect of methanol (MeOH) fixation on adult murine dentate gyrus (DG) single cell suspensions. Overall design: Freshly and methanol-fixed neuronal and non-neuronal cells from the DG of adult C57BL/6J mice were isolated by Fluorescence-activated cell sorting (FACS), according to the presence or absence of Sytox AADvanced⢠Dead Cell Stain, and analyzed using SOrting and Robot-assisted Transcriptome SEQuencing (SORT-seq), a partially robotized version of the CEL-seq2 protocol.
创建时间：
2023-11-01
