Processed single-cell RNA-seq datasets of PBMC (1k, 3k, 4k, 8k) re-aligned with Cell Ranger v9.0.1 and GENCODE v48 (GRCh38.p14)

This dataset contains processed single-cell RNA sequencing (scRNA-seq) data from peripheral blood mononuclear cells (PBMCs) with different cell counts (1k, 3k, 4k, 8k). The raw FASTQ files were downloaded from publicly available 10x Genomics datasets, and all samples were aligned using Cell Ranger v9.0.1 (February 6, 2025) with a custom-built reference genome based on GENCODE Release 48 (GRCh38.p14). For each dataset, the following files are provided: *_filtered_feature_bc_matrix.h5: filtered gene-barcode matrices *_raw_feature_bc_matrix.h5: unfiltered matrices To facilitate data interpretation, the table below summarizes key metadata for each dataset, including sample source and assay details. Dataset Number of cells Species Tissue Disease Assay pbmc1k ~1,000 Human Blood Healthy 10x 3' v3 pbmc3k ~3,000 Human Blood Healthy 10x 3' v1 pbmc4k ~4,000 Human Blood Healthy 10x 3' v2 pbmc8k ~8,000 Human Blood Healthy 10x 3' v2