Junko Y. Toshima, Jiro Toshima, Marko Kaksonen, Adam C. Martin, David S. King, David G. Drubin (2011) CIL:31918, Saccharomyces cerevisiae. CIL. Dataset

Localization on the cell surface of Alexa Fluor-488-α factor (left, green in merge) and Sla1-RFP (center; red in merge). Within each white box, α-factor spots first appear and are subsequently joined by Sla1p. The yellow boxes show colocalization of α-factor and Sla1p. These observations establish that cortical actin patches are sites of receptor-mediated α-factor internalization. Total internal reflection fluorescence (TIRF) microscopy was used to visualize A488-α factor to reduce background and Sla1-RFP was visualized by epifluorescence. Interval between frames is 2 s. Fig 1F (single frames) and Movie 3 from Toshima et al.

本数据集展示了 Alexa Fluor-488-α 因子（左侧，合并图中的绿色部分）和 Sla1-RFP（中央；合并图中的红色部分）在细胞表面上的定位。在每个白色方框内，首先出现 α 因子斑点，随后 Sla1p 蛋白与其结合。黄色方框显示了 α 因子和 Sla1p 的共定位现象。这些观察结果证实了皮质肌动蛋白斑是受体介导的 α 因子内化的位点。采用总内反射荧光（TIRF）显微镜可视化 A488-α 因子以降低背景干扰，并利用共聚焦荧光显微镜可视化 Sla1-RFP。帧间间隔为 2 秒。图 1F（单帧）和 Toshima 等人提供的电影 3。