CCALNN-PEG monolayer proportion influence on gold nanoparticles uptake (Figure S3 dataset)

HeLa cells were incubated in suspension with 5 nm diameter gold nanoparticles (final concentration 100 nM) coated with a mix of CALNN and CCALNN-PEG peptides for 10 min in serum-free medium and a further 30 min in complete medium (10 % FCS). Nanoparticles/medium were then discarded, cells transferred to a dish and left to attach for 4 h in complete medium, fixed and later imaged by photothermal microscopy. Images are two-plane TIFF images of the nanoparticles uptake by HeLa cells. The first image plane is the brightfield image, and the second image plane is the photothermal image. 0%_CCALNN-PEG_&amp;_100%_CALNN_[1-5].tif are images of cells incubated with nanoparticles coated with 100% CALNN peptides.<br>10%_CCALNN-PEG_&amp;_90%_CALNN_[1-4].tif are images of cells incubated with nanoparticles coated with 90% CALNN and 10% CCALNN–PEG peptides.<br>20%_CCALNN-PEG_&amp;_80%_CALNN_[1-4].tif are images of cells incubated with nanoparticles coated with 80% CALNN and 20% CCALNN–PEG peptides.<br>30%_CCALNN-PEG_&amp;_70%_CALNN_[1-5].tif are images of cells incubated with nanoparticles coated with 70% CALNN and 30% CCALNN–PEG peptides.