Cellular senescence and its associated AKR1B1 promote cyst growth in ADPKD

Autosomal dominant polycystic kidney disease (ADPKD) is one of the most common, life-threatening inherited kidney diseases. Cellular senescence regulates not only physiological and homeostatic processes, particularly during embryonic development and wound healing, but also pathological processes that contribute to aging, cancer, and other diseases in response to endogenous and exogenous stresses. However, the role of cellular senescence in ADPKD remains elusive. To determine whether D-gal induces differential senescence response in Pkd1-null vs. wild-type cells, we performed RNA-sequencing (RNA-seq) analysis. To determine whether D-galactose (D-gal) induces differential senescence response in Pkd1-null vs. wild-type cells, we firstly treated Pkd1 WT MEK and Pkd1 null MEK cells with D-gal (40g/l) for 48 hours and then performed RNA-sequencing (RNA-seq) analysis.