Oviposition activity index for different substrates in laboratory and semi-field conditions.

In each ovitrap tested, eggs laid in the paddle, and eggs laid in the solution or in the plastic surfaces were manually counted, separately. In each condition, the Oviposition Activity Index (OAI) was calculated considering the paddle as the experimental group and the other conditions (named as alternative surface) as the control. A separate analysis was performed grouping the data from laboratory and semi-field conditions (named “overall”). Nt: the sum number of eggs in experimental treatment; Nc: the sum of number of eggs in control; alternative surface: solution and plastic surfaces of the ovitrap; OAI: Oviposition activity index; Laboratory: assays under laboratory conditions for both 48h and 72h; Semi-field: 72h assays at environmental conditions with natural light.