Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (RNA-Seq)

we established a human intestinal stem cell (ISC) culture method expanded under feeder-free and fully defined conditions through selective enrichment of ISC populations (ISC3D-hIO) within hIO derived from human pluripotent stem cells. Single cell RNA-sequencing of human intestinal stem cells (ISCs) derived from 3D human intestinal organoids (3D HIOs)