Comparison of miRNA profiles between young (6 months) and old (24 months) male mouse vertebral samples
收藏Alliance of Genome Resources2026-08-01 收录
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To understand the effect of age on miRNA profiles in male mouse vertebrae, we isolated L4-5 vertebral samples and digested with endotoxin-free collagenase (Liberase) twice for 30 minutes. The resulting bone was confirmed to be largely osteocyte-enriched and RNA was isolated using QIAzol reagent.
