Vertebrae recovery of ovariectomy rats using fishbone flour therapy and Calcium Carbonate (CaCO3)

This research was conducted in Ternate City, North Maluku Province. The research has been approved by the Research Ethics Committee with approval No. 220-KEP-UB. This research consists of three stages: first, processing yellowfin fish bone waste into the fishbone meal with deproteinated, non-deproteinized, and calcium carbonate treatments as a comparison. The second research stage was to conduct a physicochemical analysis of the yellowfin fishbone meal. The third stage was using X-rays to confirm the degree of vertebral bone damage in ovariectomy rats treated with deproteinated, non-deproteinated fish bone meal and calcium carbonate. All statistical analyses were performed using SPSS 28.0 software[24]. The differences between groups were compared using Student's t-test, which is to analyses between boiling water and the previous one to determine the difference between acetic acid and hydrochloric acid The results of the chemical analysis carried out on yellowfin fish bone meal include moisture, ash, fat, protein, calcium, and phosphorus content. The results of the chemical analysis show that the highest value of calcium is found in the boiling treatment of yellowfin fish bones using the non-deproteination method, while the high importance for phosphorus is using deproteinated boiling.