Tobacco-related alterations in airway gene expression are rapidly reversed within weeks following smoking cessation

RNA was obtained longitudinally from normal nasal epithelium of smokers who have quit smoking over 6 months period. Statistical analysis of gene expression data identified genes differentially expressed with short-term smoking cessation and categorized the kinetics of of these genes in different biological functions with different dynamics following smoking cessation. Affymetrix GeneST arrays were run on total RNA obtained nasal epithelial brushings of 8 active smokers enrolled in smoking-cessation programs. Brushings were obtained at baseline and at 4, 8, 16, and 24-weeks after smoking-cessation (n=33). Replicates are excluded.