Quantification of mutant PTEN Binding to stBLMs by SPR.
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Dissociation constants, Kd, in µM for the binding, at room temperature, of wt PTEN to stBLMs prepared by RSE on SAMs composed of HC18∶βME 70∶30 from lipid solutions of the compositions shown. The lipid chain compositions are dioleoyl (DOPC, DOPS) and that of the natural brain extract for PI(4,5)P2 (mostly stearoyl-arachidonoyl). All experiments were evaluated as single component fits. The parameter ranges given for adsorption of the truncated PTEN PC∶PS stBLMs were estimated as described in Information S1.
创建时间:
2015-12-02



