Stability constants, K, for the binding of different organic anions to the DipA channel.

The organic anions are important key metabolites of Borrelia species. The membranes were formed from diphytanoyl phosphatidylcholine dissolved in n-decane. The buffered aqueous phase (1 mM Tris-HCl pH 7.5) contained purified DipA in a concentration of about 100 ng/ml and 0.1 M KCl; temperature = 20°C; voltage = 20 mV. The stability constants were derived from titration experiments similar to those shown in Fig. 3. The stability constant, K, is given as the mean of n experiments ± SD. Ks is the half-saturation constant.