Characterization of circulating PDGFRa positive cells in fetus

Mesenchymal stem/stromal cells (MSCs) are supplied to the damaged tissue via circulation when the injury is massive. MSCs recruited to the damaged tissues support tissue regeneration by differentiating into parenchyma cells and suppressing inflammation. However, most of the analysis of the circulating MSCs have been conducted in adult models, the existence and properties in fetal circulation was not known. Here, we have extensively analyzed the circulating MSCs cells during development. Overall design: We isolated the circulating PDGFRa positive cells as GFP positive cells using PDGFRa-GFP knockin mice. The isolated cells were cultured and analyzed by single cell RNA-seq to clarify gene expression programs.