Lymphoid subsets in the AML bone marrow microenvironment following chemotherapy induction

Using scRNA-seq, bone marrow (BM) aspirates, collected post-induction, from 12 complete responders (CR) and 10 refractory patients were compared for lymphoid cells subpopulations. Overall design: AML patients' bone marrow (BM) samples and information were collected after obtaining consent in accordance with institutional review board-approved studies at Fred Hutchinson Cancer Research Center (FHCRC). One vial of BM from each of the 22 patients was thawed with standard protocol. Then, cells were counted, and viability was evaluated with Trypan blue staining. 50,000 cells were directly sent to the Center for Medical Genomics of the Indiana University School of Medicine to be processed on the 10x Genomics while the leftover cells were stained for multicolor flow cytometry.