List of Semi-tryptic and tryptic peptides identified in 3 replicates of different digestion methods ( Urea, In-gel, FASP and RapiGest) for both CN (Centrigugation) and PN for lysosome enriched sample
Tissue proteomics is increasingly recognized for its role in biomarker discovery and disease mechanism investigation. However, protein solubility remains a significant challenge in mass spectrometry (
In this work, it was developed an alternative analytical strategy for the preparation of vegetal samples extracted with diluted acids and assisted by ultrasound and microwave radiation for the determi