Dynamic brain cell changes after middle cerebral artery occlusion revealed by massive single cell RNAseq

We used 10XGenomics Single Cell 3' Droplet-based single cell approach to profile adult mouse cortical brain cells after middle cerebral artery occlusion. Overall design: Single brain cells were isolated and profiled from two brain regions (both ipsilateral perilesional regions and its coresponding contralateral regions ) and 4 different time points after MCAO (4hrs, 1 day, 3 days and 7 days). Moreover, single cells from 3 uninjured mice coresponding regions were also being profiled.