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RNA sequencing of mouse dendritic cells in Aire-reporter strain

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NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE211006
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Bulk RNA-seq analysis of mouse dendritic cells (DCs) in Aire-reporter strain (AGF-KI: human AIRE and GFP-Flag tag knock-in). Aire+ DCs and Aire- DCs were isolated from lymph nodes and bone marrow, using Aire-sufficient (+/KI) and Aire-deficient background (-/KI). AGF+ and AGF− LNDCs were isolated from lymph node cells gated for CD45+CD11c+MHCII+ fraction. AGF+ and AGF- BMDCs were isolated from bone marrow cells gated for B220−CD24+CD11b−/low fraction and B220−CD11c+MHCII+ fraction, respectively. All samples were isolated from 6 weeks old male mice on C57BL/6 strain.
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2023-04-06
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