Optimum of PBMC cell proliferation was at 400 setae/micro-well.
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PBMC from one donor previously exposed to setae was incubated with different numbers of setae medium (0 setae), 200 setae, 400 setae and 800 setae/micro- well. Setae were suspended in PBS and sonicated before stimulation and cell proliferation was determined by 3H-thymidine incorporation. The experimental setup is described in material and methods. The mean ct/min of 4–5 parallel incubations ± standard deviation (SD) are given.Optimum of PBMC cell proliferation was at 400 setae/micro-well.
创建时间：
2015-12-02
