Comparison of the base-calling accuracy statistics of Base-Caller with Vocabulary program (BCV) and other programs.

Sequences predicted by one of the following basecallers – BCV, ABI Basecaller 3100, TraceTuner v. 3.01 and PolyScan – are compared with manually assembled sequence datasets of genome fragments of Hepatitis A (HAV) and Hepatitis D (HDV) Viruses. The reference sequences in the HAV dataset do not have ambiguous IUPAC symbols and have moderate portion (3–14% ) of SNV in the HDV dataset. The standard measures of sensitivity, specificity and identity are shown.