Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of mouse nucleus accumbens (jrc_mus-nacc-3)

<b>Sample</b>: Wild-type, adult male mouse, strain: C57/BL6J from Charles River<b>Sample Description</b>: Exemplary data used to demonstrate the capabilities of Enhanced FIB-SEM systems for large-volume 3D imaging.<b>Protocol</b>: Chemical fixation, reduced osmium processing, treated with 2% samarium trichloride and 1% uranyl acetate in maleate buffer, then embedded in Durcupan resin.<b>Contributions</b>: Sample provided by and prepared for imaging by Richard Weinberg (University of North Carolina), with imaging by C. Shan Xu (Yale), Kenneth Hayworth (HHMI/Janelia), and post-processing by Gleb Shtengel, and Harald Hess(HHMI/Janelia).<b>Acquisition ID</b>: jrc_mus-nacc-3<b>Voxel size (nm)</b>: 4 x 4 x 3.8 (X, Y, Z)<b>Data dimensions (µm)</b>:20.4 x 21.0 x 2.9 (X, Y, Z)<b>Imaging start date</b>: 2014-09-16<b>Imaging duration (days)</b>: 3<b>Landing energy (eV)</b>: 1100<b>Imaging current (nA)</b>: 0.21<b>Scanning speed (MHz)</b>: 0.403<b>Dataset URL</b>: s3://janelia-cosem-datasets/jrc_mus-nacc-3/jrc_mus-nacc-3.zarr/recon-2/em/<b>Visualization Website</b>: https://openorganelle.janelia.org/datasets/jrc_mus-nacc-3<b>Publication</b>: Wu et al., 2017: Xu et al., 2017; Xu et al., 2021