Gene 27c of pLS20cat encodes a repressor of conjugation.
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https://figshare.com/articles/dataset/_Gene_27c_of_pLS20cat_encodes_a_repressor_of_conjugation_/839195
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Conjugation kinetics of pLS20cat and pLS20rco were determined with and without ectopic expression of pLS20 gene 27c as described in Materials and Methods. PKS7 was used as recipient strain. Donor strains PKS14 (pLS20cat) and PKS86 (pLS20rco) both contain an ectopic copy of pLS20cat gene 27c under the IPTG inducible Pspank promoter at the chromosomal amyE locus. t = 0 corresponds to the end of the exponential growth phase. The conjugation efficiencies obtained for strains PKS14 and PKS86 were below the detection level of 1×10−8 when grown in the presence of IPTG.
创建时间：
2013-10-31
