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High-throughput yeast CRISPR genome editing

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NIAID Data Ecosystem2026-03-12 收录
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Here we describe a CRISPR/Cas9-based platform in S. cerevisiae for multiplexed accurate genome editing with short, trackable, integrated cellular barcodes (MAGESTIC), which employs array-synthesized guide-donor oligos for plasmid-based high-throughput editing and features genomic barcode integration to prevent plasmid barcode loss and enable robust phenotyping.

创建时间:
2021-02-04
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