Additional file 24: of Assessing the benefits of horizontal gene transfer by laboratory evolution and genome sequencing

Excel file S2. List of de novo mutations in coding regions found in sequenced clones isolated from butyric acid evolved populations. The coordinates of mutations are given in E. coli W reference genome coordinates (sheet 1) (In ' Re c Y X $$ \operatorname{Re}{\mathrm{c}}_{\mathrm{Y}}^{\mathrm{X}} $$ ', X refers to the DNA donor identity, and Y refers to the recipient identity). Mutations are annotated according to the amino acid change they cause in the encoded polypeptide sequence. Specifically, the left first three-letter abbreviation denotes the original amino acid, the following number indicates its location, and the right abbreviation denotes the substituted amino acid. ‘fs’ denotes frameshift mutations, ‘del’ denotes a deletion, and ‘*’ denotes a stop-gain mutation. We did not observed any mutations in transferred genes from the B donors in the Re c W B $$ \operatorname{Re}{\mathrm{c}}_{\mathrm{W}}^{\mathrm{B}} $$ or K donors in the Re c W K $$ \operatorname{Re}{\mathrm{c}}_{\mathrm{W}}^{\mathrm{K}} $$ clones. (XLSX 12 kb)