RNAseq data

Mice with cardiomyocyte-specific knockout of Fth gene were generated. Whole-genome gene expression analysis was performed using heart tissues obtained from normal diet-fed and high-iron diet-fed control and KO mice (n=3 per group). Total RNA was extracted using Trizol, and cDNA samples were sequenced using a HiSeq3000 sequencing system. The reference Mus musculus genome and gene information were downloaded from the National Center for Biotechnology Information (NCBI) database. Raw reads were filtered to produce high-quality clean data from which all subsequent analyses were performed.