JASMONATE-ZIM DOMAIN proteins engage Polycomb chromatin modifiers to modulate Jasmonate signaling in Arabidopsis [ChIP-Seq]

JASMONATE-ZIM DOMAIN (JAZ) proteins are key regulators in the JA signaling pathway and function to repress the expression of JA-responsive genes. We found that JAZ proteins directly interact with several chromatin-associated Polycomb proteins to mediate repressive chromatin modifications at part of JA-responsive genes and thus their transcriptional repression in Arabidopsis. Nuclei were extracted from liquid-cultured LHP1:Flag seedlings as previously described (Louwers et al., 2009), followed by chromatin immunoprecipitation with anti-Flag M2 magnetic beads (Sigma). DNA libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB), and subsequently were sequenced on NovaSeq 6000 (Illumina). Three biologically independent samples treated by 5µM JA or mock (5000x diluted ethanol) have been analyzed.