The mRNA sequencing of primary fibroblasts from the infarcted area in hearts of p16f/fPOSTN-iCre and p16f/f mice following myocardial infarction.

Twelve-month-old p16f/fPOSTN-iCre mice and p16f/f littermates were anesthetized via intraperitoneal injection of pentobarbital sodium (50 mg/kg), followed by induction of acute MI. After a 4-week observation period, comprehensive evaluations were performed on these animals. One week prior to MI induction, the p16f/fPOSTN-iCre mice received tamoxifen (50 mg/kg) dissolved in corn oil via daily intraperitoneal injection for five consecutive days to trigger genetic recombination. Three pairs of p16f/fPOSTN-iCre and p16f/f mice were euthanized via intraperitoneal injection of pentobarbital sodium (100 mg/kg). Primary cardiac fibroblasts isolated from infarcted myocardial regions underwent mRNA amplification-based transcriptome sequencing.