Order of arrival and nutrient supply alter outcomes of coinfection with two fungal pathogens

A pathogen arriving on a host typically encounters a diverse community of microbes that can shape priority effects, other within-host interactions, and infection outcomes. In plants, environmental nutrients can drive tradeoffs between host growth and defense and can mediate interactions between coinfecting pathogens. Nutrients may thus alter the outcome of pathogen priority effects for the host, but this possibility has received little experimental investigation. To disentangle the relationship between nutrient availability and coinfection dynamics, we factorially manipulated nutrient availability and order of arrival of two foliar fungal pathogens (Rhizoctonia solani and Colletotrichum cereale) on the host grass tall fescue (Festuca arundinacea) and tracked disease outcomes. Overall, C. cereale infection facilitated infection by R. solani, increasing its infection rate regardless of the order of inoculation. Additionally, simultaneous and C. cereale-first inoculations decreased plant g..., , , # Order of arrival and nutrient supply alter outcomes of coinfection with two fungal pathogens

[https://doi.org/10.5061/dryad.pg4f4qrx4](https://doi.org/10.5061/dryad.pg4f4qrx4)

## Description of the data and file structure

This project includes two data files that are used in the provided code. NA is not applicable.

plant_traits.csv - includes plant biomass, leaf N, CN, and area. See the metadata tab for a description of each data column.

leaf_survey.csv - a survey of infection on inoculated leaves across survey dates. See the metadata tab for a description of each data column.

### plant\_traits.csv

* PlantID - unique identifier
* Nutrients - N-P-K nutrient treatment (L = low (50ppm), H = high (150ppm))
* Inoculation - inoculation treatment
* Biomass - dried above ground biomass (g)
* Rhiz_inoc_date - Date of *R. solani* inoculation
* Col_inoc_date - Date of *C. cereale* inoculation
* Epichloe - presence of *Epichloë coenophiala* endophyte
* chamber - location of plant during ex...