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Data associated with, "Kinetics and spatial distribution of β-sheet development in TDP-43CTD condensate maturation "

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NHLBI Figshare2026-05-26 更新2026-07-03 收录
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All data shown in the figures of the accompanying manuscript and Supplementary Data have been uploaded. Figure 1 data: Figure 1B, tiff files of the DIC images at 1 h and 38 h. Figure 1C, excel file with the full spectra for all condensates analyzed at 1 h and 38 h. The frequency has been calibrated using a cyclohexane standard as described in the manuscript (data is provided here instead of in Supplementary Data Excel file. Figure 1D, multi-layer tiff files containing all of the frames collected for the FRAP experiment at 1 h and 38 h (requires Fiji/ImageJ to open). Figure 2 data: A single excel file containing a sheet per panel (A-C) Figure 2A, data of the background-subtracted and normalized spectra shown in the manuscript. The frequency range provided is the same as that shown in the figure. Figure 2B, the values of the amide-I intensity and the corresponding global fits. Figure 2C, the values of the amide-III first moment and the corresponding global fits Figure 3 data: A single Excel file containing a sheet per panel (A-E) Each sheet contains the x-axis, y-axis, and data of the protein density (C-H deformation), β-sheet content (amide-I), tertiary structure (amide-III), and water (bend-libration). Figure 4 data: Figure 4A, TIFF file of the DIC image showing the location of the z-slice taken on the condensate with aggregate-like morphology. A single Excel file containing a sheet per panel (B, C) Figure 4B, x-axis, z-axis, and data of the protein density (C-H deformation). Figure 4C, x-axis, z-axis, and data of the β-sheet content (amide-I). Figure 5 data: A single Excel file containing a sheet per panel (A-C) Figure 5A, the x-axis, y-axis and data of the protein density (C-H deformation) at 23 h and 39 h. Figure 5B, the x-axis, y-axis and data of the β-sheet content (amide-I) at 23 h and 39 h. Figure 5C, data for the amide-I spectral region of the ROIs highlighted in Figure 5B (r1 and r2) at 23 and 39 h. Supplementary Data: TIFF files for images shown in Figure S1, S4 and S7. A single excel file containing the data shown in Figures S3, S5, S6, and S8.

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2026-05-26
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