Percentage (and number) of amino acids in a given conformation at indicated temperature obtained from SELCON3 and CONTIN algorithms.

Estimated secondary structure percentages of amino acids in each construct at indicated temperatures according to DICHROWEB analyses. The corresponding number of amino acids is given in parentheses. While the temperature-dependent changes are predominantly alpha-helical, small changes in beta-sheet structure are also estimated. The number of residues that adopt a turn structure remains virtually unchanged. Note that the fusion proteins contain a Trx tag, which based upon the Trx crystal structure (PDB: 1F6M) has 39 and 31 amino acids that adopt an alpha-helical and a beta-sheet structure, respectively [70]. Thus, much of the alpha-helical and beta-sheet structure detected in the fusion proteins by DICHROWEB is contributed by the Trx moiety.Percentage (and number) of amino acids in a given conformation at indicated temperature obtained from SELCON3 and CONTIN algorithms.