Analysis of gene expression in USP16 knockdown PC3 prostate cancer cells. Analysis of gene expression in USP16 knockdown PC3 prostate cancer cells

Total RNA was obtained from PC3 cells with or without USP16 knockdown to perform a High-throughput sequencing. In our study, USP16 is necessary for prostate cancer cell proliferation in vitro and in vivo. Therefore, we perform the RNA-seq to explore potential mechanisms of USP16 regulating cell growth. Overall design: The prostate cancer cell line PC3 expressing shRNA targeting USP16 or a vector control. The gene transcript expression profiles were generated by deep sequencing.