Expression data from lateral nasal gland of male B6, 129/Sv and Cyp2g1-null mice

In male Cyp2g1-null mice, the lateral nasal gland, one of the largest anterior glands in the nasal cavity, was found to be protected from acetaminophen toxicity. The goal of this study was to identify the genes that are involved in the mechanisms, especially those genes functional in drug metabolism, clearance and post-activation events. Lateral nasal gland from 2-3-month-old male mice were dissected from each strain of mouse (B6, 129/Sv and Cyp2g1-null). Both B6 and 129/Sv are used as control mice because the Cyp2g1-null mice are on the mixed genetic background. Glands from 10 mice of each strain were pooled for RNA preparaion for one chip. There are totally 9 chips including 2 for 129/Sv, four for B6 and 3 for Cyp2g1-null mice, respectively.在雄性Cyp2g1敲除（Cyp2g1-null）小鼠中，鼻腔内最大的前部腺体之一——外侧鼻腺（lateral nasal gland）被证实可免受对乙酰氨基酚（acetaminophen）的毒性损伤。本研究旨在鉴定参与该保护机制的基因，尤其是在药物代谢、清除及激活后事件中发挥功能的基因。本研究从3个小鼠品系（B6、129/Sv及Cyp2g1敲除小鼠）中，分别解剖获取2~3月龄雄性小鼠的外侧鼻腺。由于Cyp2g1敲除小鼠为混合遗传背景，故选用B6与129/Sv作为对照品系。每个品系取10只小鼠的腺体混合后提取RNA，用于制备一张芯片（chip）。本次实验共制备9张芯片，其中129/Sv品系、B6品系及Cyp2g1敲除小鼠分别对应2张、4张及3张芯片。