SNPs derived from a common garden experiment across the biogeographic range of Kelletia kelletii

Signals of natural selection can be quickly eroded in high gene-flow systems, severely challenging efforts to understand how and when genetic adaptation occurs in the ocean. This long-standing, unresolved topic in ecology has renewed importance because rapidly changing environmental conditions are driving range expansions that, in many cases, necessitate rapid evolutionary responses. Kellet’s whelk expanded their biogeographic range in the 1980s and are readily adapting to novel conditions in its expanded range. To test for genetic adaptation in a coastal marine species with high dispersal potential, we performed a series of crosses on Kellet's whelk (Kelletia kelletii) collected from its historical and recently colonized range, and conducted RNA-Seq on offspring that we reared in a common garden environment. We identified 2,770 differentially expressed genes between 54 samples with historical-range and expanded-range ancestry. Using SNPs called directly from the differentially expresse..., Using SCUBA, we collected adult Kellet’s whelks by hand from sub-tidal (approximately 15 m depth) locations across California (Figure 1A, CDFW SCP #8018 to C.W.). Adult whelks from three locations: Monterey (MON, north of Point Conception; 36.6181670 N, 121.89 W), Naples (NAP, just south of Point Conception; 34.4219670 N, 119.952283 W), and Point Loma (POL, Southern California; 32.665333 N, 117.261517 W) were used in our main experimental cross (described below). Additionally adult whelks from Diablo Canyon (DIC in the expanded range; 35.22445 N, 120.877483 W) were collected and used to validate results of the main experimental cross.  
We acclimated 40 individuals each from MON, NAP, and POL in common garden aquaria using the flow-through filtered seawater system at the California Polytechnic State University Research Pier in Avila, California (35.169817 N, 120.740838 W). Additionally, an experimental reciprocal cross between MON and NAP was created with 7 individuals from both sites. ..., , # VCFs for: Genetic adaptation despite high gene flow in a range-expanding population

[https://doi.org/10.5061/dryad.qbzkh18s3](https://doi.org/10.5061/dryad.qbzkh18s3)

Included here are unfiltered VCFs files associated with the publication \"Genetic adaptation despite high gene flow in a range-expanding population\" in Molecular Ecology. SNPs are called from RNA-Seq reads of samples reared in a common garden. 

## Description of the data and file structure

We have included VCF files for both the \"All-SNPs\" and \"DEGs-SNPs\" datasets associated with the study. All-SNPs includes SNPs called across the transcriptome, and DEG-SNPs include only the SNPs found on on differentially expressed genes (DEGs) between the expanded and historical range of Kellet's whelks. 

## Sharing/Access information

Raw reads can be found in SRA: [https://www.ncbi.nlm.nih.gov/sra/PRJNA1000198](https://www.ncbi.nlm.nih.gov/sra/PRJNA1000198)

## Code/Software

[https://github.com/ChristieLab/kellets_whelk_rnaseq](...