Peptides that inhibit neuronal nitric oxide synthase

Neuronal nitric oxide synthase (nNOS) inhibition tests were carried out on peptides in addition to other bioactivity experiments, in particular, antibiotic activity. Inhibition of nNOS was measured by monitoring the conversion of [3H]arginine to [3H]citrulline.Information recorded: individual identifier for sample, peptide sequence, molecular weight, stock concentration (mg/ml), source (native peptide, synthetic derivative), solvent and concentration. To test whether the peptides inhibit nNOS. Peptides tested: aurein, caerin, citropin, cupiennin, dahlein, frenatin, lesueurin, rothein, signiferin, uperin.Sources of native peptides: Cirinia signifera; Cupiennius salei; Litoria aurea, L. caerulea, L. citropa, L. chloris, L. dahlii, L. electrica, L. gilleni, L. gracilenta, L. infrafrenata, L. lesueuri, L. rothii, L. rubella, L. splendida, hybrid L. caerulea/splendida; Uperoleia mjobergii.The first report of neuronal nitric oxide synthase inhibition by a component of a spider venom (Cupiennius salei).Subsets of the data have been used in a number of studies.