Spontaneous plasmid transformation of Escherichia coli is independent of the DNA uptake machinery for single-stranded DNA (ssDNA) entry. The one-hit kinetic pattern of plasmid transformation indicates
The pHel3 plasmid was transformed into Escherichia coli DH5a, E. coli CFT073, and Helicobacter pylori JP26. Genomic and plasmid DNA was purified from each of the three cultures and SMRT sequenced.