Spine density on the proximal and distal dendritic domains of the Purkinje cells in different experimental approaches.

Mean values of spine densities expressed as number of spines per µm of dendritic length, ±s.e.m.. In A and B, the asterisks indicate the significance between the group treated with EphA4/Fc and the control, performed with the Student's t-test. In C, the asterisk indicates the significance between the group treated with EphA4/Fc, the control and the negative control Fc, performed with one way ANOVA followed by the post hoc Student-Newman-Keuls method. In D and E, pairwise multiple comparisons between groups were performed with one way ANOVA followed by the post hoc Student-Newman-Keuls method. The asterisk in D indicate a statistically significant difference between the latter group and the others, whereas in E the post hoc method indicates a significant difference between the groups treated with ligand chimeras in addition to TTX and those treated with TTX and Fc+TTX, but not between each other. Abbreviations used: eA2, ephrinA2/Fc; eB1, ephrinB1/Fc; EA4, EphA4/Fc; Fc, portion of recombinant human IgG1 (negative control); TTX, tetrodotoxin.